SLC31A2 Knockout cell line (HCT 116)

SLC31A2 Knockout cell line (HCT 116) Order

Catalog#

YKO-XN23075

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

SLC31A2 Knockout cell line (HCT 116)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

SLC31A2

Gene ID

1318

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%McCOY's 5A+10% FBS

Target region

Knockout region: Exon 2 (based on transcript SLC31A2-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SLC31A2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SLC31A2 gene knockout SLC31A2 Knockout cell line (HCT 116) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

SLC31A2 Knockout cell line (HCT 116)

Catalog#

YKO-XN23075

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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SLC31A2 Knockout cell line (HCT 116)

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